CD103 (Integrin alpha E) Monoclonal Antibody (2E7), eFluor 450, eBioscience

PRODUCT DETAILS

Host: Armenian Hamster

Isotype: IgG

Clonality: Monoclonal

Clone: 2E7

Format: eFluor 450

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: 1 µg/test

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta7 and is expressed on all intraepithelial lymphocytes, a small subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC). Epithelial cell antigen E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.

Applications Reported: This 2E7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2E7 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.