CD103 (Integrin alpha E) Monoclonal Antibody (Ber-ACT8), APC, eBioscience

PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: Ber-ACT8

Format: APC

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 5 µL (0.06 µg)/test

Concentration: 5 μL/Test

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The Ber-ACT8 monoclonal antibody reacts with human CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. \I{In vitro\I} stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen (E-cadherin) binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium. Cross-blocking studies suggest that antibody clones B-Ly7 and Ber-ACT8 see different epitopes.

Applications Reported: This Ber-ACT8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Ber-ACT8 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.