CD162 (PSGL-1) Monoclonal Antibody (4RA10), PE-eFluor 610, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 4RA10
Format: PE-eFluor 610
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.06 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 4RA10 monoclonal antibody reacts with mouse CD162, which is also known as P-selectin glycoprotein ligand-1 (PSGL-1). This clone has been successfully used in flow cytometry and has been reported to block binding of CD162 to CD62P and CD62L.
Applications Reported: This 4RA10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 4RA10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $472.00
-70%$472.00
$141.60
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 4RA10
Format: PE-eFluor 610
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.06 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This 4RA10 monoclonal antibody reacts with mouse CD162, which is also known as P-selectin glycoprotein ligand-1 (PSGL-1). This clone has been successfully used in flow cytometry and has been reported to block binding of CD162 to CD62P and CD62L.
Applications Reported: This 4RA10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 4RA10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
