CD19 Monoclonal Antibody (eBio1D3 (1D3)), eBioscience

PRODUCT DETAILS

Host: Rat

Isotype: IgG2a, kappa

Clonality: Monoclonal

Clone: eBio1D3 (1D3)

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: 0.5 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The eBio1D3 monoclonal antibody has also been reported to induce down-regulation of CD19, affecting the proportions of B-1a and B-2 B cells in mice. (Please use Functional Grade purified eBio1D3 (1D3), Product # 16-0193, in functional assays).

Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.