CD1a Monoclonal Antibody (HI149), eBioscience

PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: HI149

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 1 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells. The CD1 family of proteins share some structural and functional characteristics with the MHC class I molecules; however, members of the CD1 family are not polymorphic. Similar to MHC class I, CD1a associates with the beta2-microglobulin and is thought to play a role in antigen presentation.

Applications Reported: The HI149 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested: This HI149 antibody has been tested by flow cytometric analysis of human MOLT-4 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.