CD204 Monoclonal Antibody (M204PA), Alexa Fluor 488, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: M204PA
Format: Alexa Fluor 488
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This M204PA antibody recognizes mouse CD204 also known as Macrophage Scavenger Receptor 1 (MSR 1), Scavenger Receptor A (SR-A) or SCARA1. CD204 belongs to the class A family of scavenger receptors that are primarily responsible for binding a wide variety of macromolecules and are involved in many aspects of macrophage activity. Other members of this family include: MARCO (SCARA2), CSR (SCARA3), SRCL (SCARA4) and SCARA5. All class A scavenger receptors are trans-membrane proteins with extracellular C-termini and homotrimeric structures. CD204 exists as 2 splice variants: the more ubiquitous full length isoform (type I) and a shorter isoform (type II) that lacks the C-terminal cysteine-rich domain. Both types are functional and bind modified LDL with similar affinity. In addition to lipid metabolism, CD204 plays an important role suppressing inflammation and macrophage activation. CD204 is considered one of the markers of M2 and Tumor-Associated Macrophages. Interestingly, this molecule is responsible for adherence of RAW264.7 cells to tissue culture-treated plastic in the absence of Ca and Mg cations.
This M204PA antibody binds to CD204 of multiple mouse strains, including C57Bl/6, Balb/c and Swiss-Webster (outbred). This antibody works on fixed cells and can be used to stain CD204 intracellularly. The M204PA antibody does not cross-react with human or rat. This antibody is not suitable for IHC staining.
Applications Reported: This M204PA antibody has been reported for use in flow cytometric analysis.
Applications Tested: This M204PA antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells.This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, kappa
Clonality: Monoclonal
Clone: M204PA
Format: Alexa Fluor 488
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This M204PA antibody recognizes mouse CD204 also known as Macrophage Scavenger Receptor 1 (MSR 1), Scavenger Receptor A (SR-A) or SCARA1. CD204 belongs to the class A family of scavenger receptors that are primarily responsible for binding a wide variety of macromolecules and are involved in many aspects of macrophage activity. Other members of this family include: MARCO (SCARA2), CSR (SCARA3), SRCL (SCARA4) and SCARA5. All class A scavenger receptors are trans-membrane proteins with extracellular C-termini and homotrimeric structures. CD204 exists as 2 splice variants: the more ubiquitous full length isoform (type I) and a shorter isoform (type II) that lacks the C-terminal cysteine-rich domain. Both types are functional and bind modified LDL with similar affinity. In addition to lipid metabolism, CD204 plays an important role suppressing inflammation and macrophage activation. CD204 is considered one of the markers of M2 and Tumor-Associated Macrophages. Interestingly, this molecule is responsible for adherence of RAW264.7 cells to tissue culture-treated plastic in the absence of Ca and Mg cations.
This M204PA antibody binds to CD204 of multiple mouse strains, including C57Bl/6, Balb/c and Swiss-Webster (outbred). This antibody works on fixed cells and can be used to stain CD204 intracellularly. The M204PA antibody does not cross-react with human or rat. This antibody is not suitable for IHC staining.
Applications Reported: This M204PA antibody has been reported for use in flow cytometric analysis.
Applications Tested: This M204PA antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells.This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
