CD3/CD4 Antibody Cocktail, FITC, PE, eBioscience

PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Cocktail

Clone: UCHT1, RPA-T4

Format: PE/FITC

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 20 µL/test

Concentration: 20 μL/Test

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: This product consists of two fluorochrome-conjugated monoclonal antibodies to distinguish human peripheral CD3^+ and CD4^+ T cells. The UCHT1 monoclonal antibody (mouse IgG1, kappa) reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The RPA-T4 monoclonal antibody (mouse IgG1, kappa) reacts with the human CD4 molecule, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV).

Applications Reported: This product has been reported for use in flow cytometric analysis.

Applications Tested: This cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test (containing 1 µg of Anti-Human CD3 (UCHT1) FITC and 0.5 µg of Anti-Human CD4 (RPA-T4) PE). A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.