CD3 Monoclonal Antibody (17A2), eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: 17A2
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.
Applications Reported: The 17A2 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro activation of mouse T cells. (Please use Functional Grade purified 17A2, Product # 16-0032 , in functional assays).
Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2b, kappa
Clonality: Monoclonal
Clone: 17A2
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.
Applications Reported: The 17A2 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro activation of mouse T cells. (Please use Functional Grade purified 17A2, Product # 16-0032 , in functional assays).
Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
