CD30 Monoclonal Antibody (mCD30.1), PE, eBioscience

PRODUCT DETAILS

Host: Armenian Hamster

Isotype: IgG

Clonality: Monoclonal

Clone: mCD30.1

Format: PE

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: 1 µg/test

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The mCD30.1 monoclonal antibody reacts with mouse CD30, a 105-120 kDa member of the TNFR superfamily. CD30 is not detectable on splenocytes, but can be upregulated on T cells stimulated in vitro through TCR with a peak expression around day 4-5 post-activation. CD30 is expressed by both CD8^+ and CD4^+ activated cells. It is suggested that CD30 regulates cytokine secretion by T cells and has a role in T cell development.

Applications Reported: The mCD30.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The mCD30.1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.