CD32 Monoclonal Antibody (6C4 (CD32)), Biotin, eBioscience

PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: 6C4 (CD32)

Format: Biotin

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 0.5 µg /test

Concentration: 0.5 mg/mL

Storage: 4°C, do not freeze

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: This 6C4 monoclonal antibody reacts with human CD32 (also known as Fc gammaRII). This antibody recognizes two isoforms of the receptor, Fc gammaRIIA and Fc gammaRIIB. These 40-kDa polymorphic transmembrane glycoproteins are expressed on B cells, granulocytes, monocytes, macrophages, and platelets. Moreover, these receptors are detected on natural killer cells. CD32 enables interaction between Fc gammaRII-expressing cells and opsonized antigen or IgG-containing immune complexes. Both receptors exhibit low affinity towards IgG and play a role in inflammation and autoimmune disease. This clone has been reported to inhibit Ig binding in a rosette blocking assay.

Applications Reported: This 6C4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 6C4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.