CD4/CD25 Antibody Cocktail, APC, FITC, eBioscience

PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Cocktail

Clone: RPA-T4, BC96

Format: APC/FITC

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 20 µL/test

Concentration: 20 μL/Test

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: This product consists of two fluorochrome-conjugated monoclonal antibodies to identify CD4+CD25+ human peripheral blood cells. The RPA-T4 monoclonal antibody (mouse IgG1, kappa) reacts with human CD4, a 59 kDa cell surface receptor that is expressed by a majority of thymocytes, a subpopulation of mature T cells (T helper cells) and at low levels on monocytes. The BC96 monoclonal antibody (mouse IgG1, kappa) reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). This cocktail of RPA-T4 and BC96 can be used for the flow cytometric detection of human regulatory T cells, which also express Foxp3.

Applications Reported: This product has been reported for use in flow cytometric analysis.

Applications Tested: This cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test (containing 1 µg of Anti-Human CD4 (RPA-T4) FITC and 0.125 µg of Anti-Human CD25 (BC96) APC). A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.