CD75 Monoclonal Antibody (LN-1), eFluor 660, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgM
Clonality: Monoclonal
Clone: LN-1
Format: eFluor 660
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.25 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This LN-1 monoclonal antibody reacts with human CD75, a cell surface carbohydrate determinant produced by alpha-2,6-sialytransferase. CD75 is expressed predominantly on mature B cells in peripheral blood and germinal centers, as well as several B cell lines (e.g., Raji, Ramos). CD75 is also expressed on non-immune tissues such as breast, prostate, colon, liver, and kidney. Reports demonstrate that CD75 is a ligand of CD22.
Studies have shown that, depending on fixation and tissue processing, the LN-1 antibody also recognizes astrocytes and microglia in the human brain.
Applications Reported: This LN-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This LN-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgM
Clonality: Monoclonal
Clone: LN-1
Format: eFluor 660
Reactivity: Hu
Application: Flow Cytometry
Tested Dilution: 5 µL (0.25 µg)/test
Concentration: 5 μL/Test
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: This LN-1 monoclonal antibody reacts with human CD75, a cell surface carbohydrate determinant produced by alpha-2,6-sialytransferase. CD75 is expressed predominantly on mature B cells in peripheral blood and germinal centers, as well as several B cell lines (e.g., Raji, Ramos). CD75 is also expressed on non-immune tissues such as breast, prostate, colon, liver, and kidney. Reports demonstrate that CD75 is a ligand of CD22.
Studies have shown that, depending on fixation and tissue processing, the LN-1 antibody also recognizes astrocytes and microglia in the human brain.
Applications Reported: This LN-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This LN-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
