CD86 (B7-2) Monoclonal Antibody (24F), PE, eBioscience
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 24F
Format: PE
Reactivity: Rt
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 24F monoclonal antibody reacts with rat CD86, an ~90 kDa surface receptor, also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed by germinal center B cells and at low levels on macrophages, dendritic cells and non-lymphoid cells in the thymus. Its expression is upregulated on B cells through a variety of surface stimuli including LPS. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.
Applications Reported: The 24F antibody has been reported for use in flow cytometric analysis.
Applications Tested: The 24F antibody has been tested by flow cytometric analysis of resting and activated rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Description
PRODUCT DETAILS
Host: Mouse
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: 24F
Format: PE
Reactivity: Rt
Application: Flow Cytometry
Tested Dilution: 0.25 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The 24F monoclonal antibody reacts with rat CD86, an ~90 kDa surface receptor, also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed by germinal center B cells and at low levels on macrophages, dendritic cells and non-lymphoid cells in the thymus. Its expression is upregulated on B cells through a variety of surface stimuli including LPS. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.
Applications Reported: The 24F antibody has been reported for use in flow cytometric analysis.
Applications Tested: The 24F antibody has been tested by flow cytometric analysis of resting and activated rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
