CD95 (APO-1/Fas) Monoclonal Antibody (APO-1-1), FITC, eBioscience

PRODUCT DETAILS

Host: Mouse

Isotype: IgG1

Clonality: Monoclonal

Clone: APO-1-1

Format: FITC

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: Assay-Dependent

Concentration: 10 μL/Test

Storage: 4°C, store in dark

Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.2-7.4

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: Immunohistochemistry:

APO-1-1 (BMS151, IgG1) is an isotype-switch variant of the original clone APO-1-3 (IgG3). Since undesired side reactions in immunohistochemistry are minimal for IgG1 antibodies, BMS151 is especially useful for immunohistology on cryostat sections and on cytospin preparations.

Flow cytometry:

BMS151 is also suitable as primary antibody in staining for FACS analysis, BMS151FI can be used for direct staining of cells, BMS151BT can be used in a two-step procedure using the biotin-(strept-)avidin system. Recommended concentration for the unlabelled antibody is 1 - 2 µg/mL and a 1:20 dilution of the labelled antibodies, as determined on SKW6.4 cells.

Applications Tested: Flow Cytometry.

Purity: >95%.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.