ROR gamma (t) Monoclonal Antibody (B2D), APC, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: B2D
Format: APC
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.
The ROR gamma t isoform differs from ROR gamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the ROR gamma and ROR gamma t isoforms.
Applications Reported: This B2D antibody has been reported for use in flow cytometric analysis.
Applications Tested: This B2D antibody has been tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $568.00
-70%$568.00
$170.40
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG1, kappa
Clonality: Monoclonal
Clone: B2D
Format: APC
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 1 µg/test
Concentration: 0.2 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.
The ROR gamma t isoform differs from ROR gamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the ROR gamma and ROR gamma t isoforms.
Applications Reported: This B2D antibody has been reported for use in flow cytometric analysis.
Applications Tested: This B2D antibody has been tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
