TCR beta Monoclonal Antibody (H57-597), NovaFluor UV 765, eBioscience
PRODUCT DETAILS
Host: Armenian Hamster
Isotype: IgG
Clonality: Monoclonal
Clone: H57-597
Format: NovaFluor UV 765
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.4 µg/test
Concentration: 0.1 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR beta expressing T cells.
Applications Reported: The H57-597 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The H57-597 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.4 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 358nm; Emission: 763 nm; Laser: 349 nm (Ultraviolet) Laser
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Original: $192.00
-70%$192.00
$57.60
Description
PRODUCT DETAILS
Host: Armenian Hamster
Isotype: IgG
Clonality: Monoclonal
Clone: H57-597
Format: NovaFluor UV 765
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.4 µg/test
Concentration: 0.1 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR beta expressing T cells.
Applications Reported: The H57-597 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The H57-597 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.4 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 358nm; Emission: 763 nm; Laser: 349 nm (Ultraviolet) Laser
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
