TCR V alpha 2 Monoclonal Antibody (B20.1), Biotin, eBioscience
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, lambda
Clonality: Monoclonal
Clone: B20.1
Format: Biotin
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. This antibody recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, V delta 8 due to the high degree of homology.
Applications Reported: This B20.1 antibody has been reported for use in flow cytometric analysis and immunoprecipitation.
Applications Tested: This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to preblock Fc receptors for 10 minutes using 0.5 µg of purified anti-mouse CD16/32 (Product # 14-0161-85). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Description
PRODUCT DETAILS
Host: Rat
Isotype: IgG2a, lambda
Clonality: Monoclonal
Clone: B20.1
Format: Biotin
Reactivity: Ms
Application: Flow Cytometry
Tested Dilution: 0.125 µg/test
Concentration: 0.5 mg/mL
Storage: 4°C, store in dark, DO NOT FREEZE!
Formulation: PBS with 0.09% sodium azide; pH 7.2
Purification: Affinity chromatography
Data Sheet: TDS
Specific Information
Description: The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. This antibody recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, V delta 8 due to the high degree of homology.
Applications Reported: This B20.1 antibody has been reported for use in flow cytometric analysis and immunoprecipitation.
Applications Tested: This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to preblock Fc receptors for 10 minutes using 0.5 µg of purified anti-mouse CD16/32 (Product # 14-0161-85). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
